MicroinjectionJuan Carlos Lacal, Rosario Perona, James Feramisco Springer Science & Business Media, 1 трав. 1999 р. - 248 стор. This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system. |
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Microinjection: XD-US. ... Juan Carlos Lacal,Rosario Perona,James Feramisco Обмежений попередній перегляд - 1999 |
Загальні терміни та фрази
2-deoxyglucose-6-P antibody aphidicholin apoptosis arrest assay binding Biochem Biol Chem blocking solution BrdU buffer Solution c-myc Ca2+ entry capillary Carnero Cdc42Hs cDNA Cell Biol cell cycle cellular centrifugation cloning concentration coverslips culture cyclin cytes cytoplasmic dehydrogenase EMBO embryos endogenous enzyme expression plasmid factor Feramisco fibroblasts fluorescence functional gene glucose transport glucose-6-P glycogen synthase Graessmann GTPase GVBD heparin incubate induced inhibit inhibitor injected cells intracellular jected Lacal LTAg mammalian cells MAP kinase MAPK measured meiosis meiotic membrane mg/ml microin microinjection microscope microtubule Molecular Mos protein mouse mRNA mutant myocytes needle nematode ng/ml nuclear oncogene oocyte maturation oocytes pathway PDGF peptide peroxisomal phosphorylation pipet plasmid progesterone protein kinase Protocol Ras proteins reaction Reagent receptor regulation S-phase Sagata sample serum Sigma signal specific staining substrate subunit synthesis tein tion vitro Wash cells Xenopus laevis Xenopus oocytes µg/ml
Популярні уривки
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